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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is noticed in lots of tumors, which ends up in chromosome instability, and thus might contribute to tumorigenesis. This protein was located to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential function in selling mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are explained. Two supplemental splice variants were documented but in-depth nucleotide sequence info is just not nonetheless accessible.
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Blocking peptides are peptides that bind especially for the target antibody and block antibody binding. These peptide ordinarily has the epitope recognized by the antibody.
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This manual outlines further more doable optimization of cellular and immunoassay parameters to be certain the absolute best benefits are...
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This guideline outlines more probable optimization of cellular and immunoassay parameters to make certain the very best results are...
Antibodies certain to the blocking peptide no more bind into the epitope on the target protein. By comparing the staining in the blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Information Cyclin E belongs to the highly conserved cyclin family members, whose associates are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates with the G1-S stage boundary and is degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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